biological source
mouse adipose tissue
packaging
tube of 5 μg 11112201-DNA-5UG, pkg of vial of cells 11112201-1VL
growth mode
Adherent
karyotype
Normal diploid
morphology
Fibroblast-like
receptors
Sca-1, c-kit/CD117, nestin, nucleostemin, CD44 and CD106
Biochem/physiol Actions
Adipose cell line, mouse subcutaneous, mesenchymal, multipotent
Not specified
m17.ASC is a spontaneously immortalized multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.
Preparation Note
Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.