m17.ASC

Adipose cell line, mouse subcutaneous, mesenchymal, multipotent

Not specified

m17.ASC is a spontaneously immortalized multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.

51800C (51800C) + L-Glutamine (G7513) + FBS / FCS (F2442).

Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 10⁴ cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

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