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About This Item
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgM
Technique(s):
cell culture | mammalian: suitable
Myeloma:
P3-NS1/1-Ag4.1
Reactivity:
Human ′A′ erythrocytes
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 13082201-DNA-5UG, pkg of vial of cells 13082201-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
P3-NS1/1-Ag4.1
Reactivity
Human ′A′ erythrocytes
technique(s)
cell culture | mammalian: suitable
isotype
IgM
shipped in
dry ice
storage temp.
−196°C
Immunogen
Human tonsil membrane
Application
Human erythrocyte marker.
Biochem/physiol Actions
The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Seed at 5 x 104 cells/ml. Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. Start in 20% FBS when recovering from frozen.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Regulatory Information
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