产品名称
CHO-K1.Cl-0205, from hamster ovary, 15042905
biological source
hamster ovary
description
CHO-K1, FcγRIIa, Fc gamma receptor 2A, 131R, allotype
growth mode
Adherent
morphology
Polygonal
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Clone of CHO-K1 cells expressing human FcγRIIa of the 131H allotype as a transmembrane protein with its native intracellular domain
Stably transfected with a pcDNA3.1/Hygro(+) based vector with a human FcγRIIa 131H cDNA insert that is expressed as a transmembrane receptor
Preparation Note
MEM (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 10% FBS (F2442) with 600ug/ml hygromycin B
Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO2/95%air; 37°C
Other Notes
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.CI-0205 (ECACC 15042905)
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.