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VERO Cell Line

from African green monkey kidney, 84113001, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Synonym(s):
Vero 81 Cells, VeroCCL81 Cells

biological source

African green monkey kidney

growth mode

Adherent

karyotype

2n = 60, modal no. 58

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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General description

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

Cell Line Origin

Monkey African Green kidney

Cell Line Description

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

Application

VERO cell lines have been used:
  • in the propagation of Chandipura virus (CHPV)
  • in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
  • for the propagation of T.cruzi
Virus studies: polio, rubella, arboviruses and reoviruses

Culture Medium

(D6546) + 2 mM (G7513) + 10% (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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