HeLa Ohio
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84121901, human cervix, Epithelial
biological source
human cervix
packaging
tube of 5 μg 84121901-DNA-5UG
pkg of vial of cells 84121901-1VL
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: poliovirus 1 and Ad 3
Biochem/physiol Actions
Derivative of parent HeLa line (Sigma catalogue no. 93021013).
Human cervix carcinoma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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