MDCK
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011435, dog kidney, Epithelial-like
Synonym(s):
MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
biological source
dog kidney
packaging
tube of 5 μg 85011435-DNA-5UG
pkg of vial of cells 85011435-1VL
growth mode
Adherent
karyotype
2n = 78
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: SVEV, VSV, Vaccinia, reoviruses and inf. canine hepatitis virus
Biochem/physiol Actions
Canine Cocker Spaniel kidney
Derived from the kidney of a normal adult Cocker Spaniel in 1958. Useful in viral studies, supports the growth of vesicular exanthema virus of swine, vesicular stomatitis, vaccina, coxsackie B-5, adenoviruses, reoviruses and infectious canine hepatitis. Also known as MDCK (NBL-2).
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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