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U937 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011440, human lung (lymphoblast), Lymphoblast

Synonym(s):

U-937 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human lung (lymphoblast)

packaging

tube of 5 μg 85011440-DNA-5UG
pkg of vial of cells 85011440-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity studies
U937 Cell Line human has been used to study the toxicity of propolis extracts against U937 cells and to study the uptake of apoptotic bodies (derived from monocytic U-937 cells) by primary meningothelial cells (PMECs).

Biochem/physiol Actions

Derived from malignant cells of a pleural effusion of 37 year old caucasian male with diffuse histiocytic lymphoma. One of only a few human lines still expressing many of the monocytic like characteristics exhibited by cells of histiocytic origin.
Human Caucasian histiocytic lymphoma
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 14,15

Preparation Note

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C. Cells may take up to 72 hours until confluent.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  6. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  7. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

  8. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

  9. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  10. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

  11. How to freeze down Cell Line cells to make a cell stock?

    A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

  12. Are the Cell Line cells tested for viral pathogens?

    HPA Cultures does not perform any viral testing on the cell lines.

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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