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RAMOS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85030802, human lymph, Lymphoblast

Synonym(s):

RA 1 Cells, Ramos (RA 1) Cells, Ramos 1 Cells, Ramos-RA1 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

human lymph

description

Human Caucasian Burkitt′s lymphoma

packaging

tube of 5 μg 85030802-DNA-5UG
pkg of vial of cells 85030802-1VL

growth mode

Suspension

karyotype

Modal no. 45

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity and virus studies: Epstein-Barr virus (EBV).
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation.

Biochem/physiol Actions

Derived from a Burkitt′s lymphoma which does not possess the EBV genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.
Human Caucasian Burkitt′s lymphoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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