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About This Item
UNSPSC Code:
41106514
Biological source:
mouse nerve
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Glial
biological source
mouse nerve
packaging
tube of 5 μg 85042301-DNA-5UG, pkg of vial of cells 85042301-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Glial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Ethyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.
Mouse C3H.He Schwannoma
Preparation Note
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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