Do-11-10
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85082301, mouse unknown/unspecified, Not specified
biological source
mouse unknown/unspecified
packaging
tube of 5 μg 85082301-DNA-5UG
pkg of vial of cells 85082301-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.
Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147
Preparation Note
DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.
Other Notes
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Regulatory Information
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