NIH-3T3D4
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
mouse embryo
packaging
tube of 5 μg 85111801-DNA-5UG
pkg of vial of cells 85111801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
Product has lost contact inhibition
Biochem/physiol Actions
Derived from the NIH-3T3 cell line. No longer exhibit contact inhibition.
Mouse Swiss NIH embryo
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2x10,000 to 2x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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