SK.N.SH Cell Line human

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

Human Caucasian neuroblastoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.