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MG-63 Cell Line human

86051601

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Synonym(s):
MG63 Cells

biological source

human bone

description

Human osteosarcoma

growth mode

Adherent

karyotype

Modal no. 66; hypotriploid

morphology

Fibroblast

products

Inducible interferon

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human osteosarcoma

Cell Line Description

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

Application

Human MG-63 cell line has been used:
  • to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells
  • to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells
  • as a positive control for immunostaining procedure
  • to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells
Interferon-induction studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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