Hs 578T

Hs 578T has been used to measure the calcium-dependent binding of cadherin-11 and N-cadherin. It has also been used to study the changes in gene expression induced by insulin-like growth factor-binding protein (IGFBP-2).

Human breast carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17

Tumorigenic in immunosuppressed mice and form colonies in semisolid medium. Neither oestrogen receptors nor endogenous viruses were detected.

DMEM + 2mM Glutamine + 10μg/ml Bovine Insulin + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.