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About This Item
UNSPSC Code:
41106514
Biological source:
bovine cardiovascular
Growth mode:
Adherent
Karyotype:
2n = 60, diploid
Morphology:
Endothelial
biological source
bovine cardiovascular
packaging
tube of 5 μg 86111401-DNA-5UG, pkg of vial of cells 86111401-1VL
growth mode
Adherent
karyotype
2n = 60, diploid
morphology
Endothelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Enzymatic studies
Biochem/physiol Actions
Bovine pulmonary artery endothelium
Established from the main stem pulmonary artery of a young, female cow (Bos taurus). The cells have angiotensin-converting enzyme activity. BVDV positive.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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