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About This Item
UNSPSC Code:
41106514
Biological source:
bovine cardiovascular
Growth mode:
Adherent
Karyotype:
2n = 60, diploid
Morphology:
Endothelial
biological source
bovine cardiovascular
packaging
tube of 5 μg 86111401-DNA-5UG, pkg of vial of cells 86111401-1VL
growth mode
Adherent
karyotype
2n = 60, diploid
morphology
Endothelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Enzymatic studies
Biochem/physiol Actions
Bovine pulmonary artery endothelium
Established from the main stem pulmonary artery of a young, female cow (Bos taurus). The cells have angiotensin-converting enzyme activity. BVDV positive.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
动物来源培养基
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