ZR-75-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012601, human breast, Epithelial
biological source
human breast
packaging
tube of 5 μg 87012601-DNA-5UG
pkg of vial of cells 87012601-1VL
growth mode
Adherent
karyotype
2n = 46, modal no. 72
morphology
Epithelial
products
Not specified
receptors
Reported to express the oestrogen and progesterone receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone and tumourigenicity studies
ZR-75-1 has been used to determine the effects of cucurbitacin E (CuE) extracted from Ecballium elaterium A.Rich., Fam. Cucurbitaceae.
Biochem/physiol Actions
Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The cells are reported to express both the wildtype and variant oestrogen receptors, progesterone receptor and other steroid hormones.
Human Caucasian breast carcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Preparation Note
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow very slowly, do not form a confluent monolayer and attach only lightly to the substrate. After subculture, it can take up to 7 days before cells re-attach. Detachment of cells can occur in PBS and trypsinisation can produce clumps of cells. To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. When subculturing, ensure the trypsin is completely removed by centrifuging the cells and resuspending them in fresh medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
高风险级别生物产品--人源产品
动植物来源培养基
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