Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
41106514
Biological source:
rat brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Hyperdiploid, modal no. 60
Morphology:
Epithelial
biological source
rat brain
packaging
tube of 5 μg 87012603-DNA-5UG, pkg of vial of cells 87012603-1VL
growth mode
Adherent
karyotype
Hyperdiploid, modal no. 60
morphology
Epithelial
products
Growth hormone, prolactin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Rat Wistar-Furth pituitary tumour
Preparation Note
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service