GH3
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012603, rat brain, Epithelial
biological source
rat brain
packaging
tube of 5 μg 87012603-DNA-5UG
pkg of vial of cells 87012603-1VL
growth mode
Adherent
karyotype
Hyperdiploid, modal no. 60
morphology
Epithelial
products
Growth hormone, prolactin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Rat Wistar-Furth pituitary tumour
Preparation Note
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask
Other Notes
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