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Merck
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WEHI 164 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87022501, mouse skin (fibroblast), Fibroblast

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

mouse skin (fibroblast)

packaging

tube of 5 μg 87022501-DNA-5UG
pkg of vial of cells 87022501-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Tumourigenicity and cytotoxicity studies, chromium release assays

Biochem/physiol Actions

Established from a fibrosarcoma induced by subcutaneous injection of methylcholanthrene in BALB/c mice. Highly sensitive after pre-treatment with actinomycin D to human cytotoxic monocytes, to tumour necrosis factor and to lymphotoxin. It provides convenient target cells for short-term (51) chromium release assays.
Mouse BALB/c fibrosarcoma

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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