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About This Item
UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, triploid
Morphology:
Epithelial
biological source
human colon
packaging
tube of 5 μg 87071008-DNA-5UG, pkg of vial of cells 87071008-1VL
growth mode
Adherent
karyotype
2n = 46, triploid
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.
Human Caucasian colon adenocarcinoma
Preparation Note
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; No CO2; 37°C. Cells do not reach confluency but will grow in small islands.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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