IEC 6
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88071401, rat intestine, Epithelial
biological source
rat intestine
packaging
tube of 5 μg 88071401-DNA-5UG
pkg of vial of cells 88071401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Small bowel biology
Biochem/physiol Actions
Normal rat small intestine epithelial cells which synthesise fibronectin and collagen. Growth is inhibited by cortisol. Cells possess cell surface antigens specific for intestinal epithelial cells in vivo. Capable of at least 10 population doublings.
Rat small intestine epithelial
Preparation Note
DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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