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About This Item
UNSPSC Code:
41106514
Biological source:
rat intestine
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
rat intestine
packaging
tube of 5 μg 88071401-DNA-5UG, pkg of vial of cells 88071401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Small bowel biology
Biochem/physiol Actions
Normal rat small intestine epithelial cells which synthesise fibronectin and collagen. Growth is inhibited by cortisol. Cells possess cell surface antigens specific for intestinal epithelial cells in vivo. Capable of at least 10 population doublings.
Rat small intestine epithelial
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
低风险生物材料
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