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H9C2 (2-1) Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88092904, rat cardiovascular, Myoblast

Synonym(s):

H9c2 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

rat cardiovascular

packaging

tube of 5 μg 88092904-DNA-5UG
pkg of vial of cells 88092904-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Myoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Differentiation studies

Biochem/physiol Actions

Rat BDIX heart myoblast
Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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