biological source
fish colon (posterior)
packaging
tube of 5 μg 88102401-DNA-5UG, pkg of vial of cells 88102401-1VL
growth mode
Adherent
karyotype
Modal no. 51
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff′s frog viruses
Biochem/physiol Actions
Derived from tissue posterior to the anus of normal adult minnows. Reported to be capable of growth over a wide temperature range from 4°C-36°C with maximum growth at 34°C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.
Fish Fat Head minnow
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3; 34°C. Dislodge cells by agitation and scraping. Cells are reported to multiply over a wide range of temperatures 0°-36°C, with maximum growth at about 34°C. It is recommended that cultures are grown at 34
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.