biological source
mouse testis
packaging
tube of 5 μg 89031605-DNA-5UG
pkg of vial of cells 89031605-1VL
growth mode
Adherent
karyotype
2n = 40, hyperdiploid
morphology
Epithelial
products
Steroids
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).
Mouse BALB/c Leydig cell testicular tumour
Preparation Note
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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