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SAOS-2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89050205, human bone, Epithelial-like

Synonym(s):

SAOS2 Cells

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About This Item

UNSPSC Code:
41106514
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biological source

human bone

packaging

tube of 5 μg 89050205-DNA-5UG
pkg of vial of cells 89050205-1VL

growth mode

Adherent

karyotype

2n = 46, (P1) Hyperploid to hypopentaploid

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.

Biochem/physiol Actions

Human primary osteogenic sarcoma
Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18

Preparation Note

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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