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About This Item
UNSPSC Code:
41106514
Biological source:
human mouth
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human mouth
packaging
tube of 5 μg 89062002-DNA-5UG, pkg of vial of cells 89062002-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Epidermal keratins; 40 kD Keratin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.
Human tongue squamous carcinoma
Preparation Note
DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone
Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm2 using trypsin/EDTA; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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