CCRF-SB
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human blood
packaging
tube of 5 μg 89090405-DNA-5UG
pkg of vial of cells 89090405-1VL
growth mode
Suspension
karyotype
2n = 46
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
HLA profile studies
Biochem/physiol Actions
A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.
Human Caucasian acute lymphoblastic leukaemia
Preparation Note
Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO2; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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