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About This Item
UNSPSC Code:
41106514
Biological source:
mouse nerve
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Unstable
Morphology:
Neuroblast
biological source
mouse nerve
packaging
tube of 5 μg 89121405-DNA-5UG, pkg of vial of cells 89121405-1VL
growth mode
Adherent
karyotype
Unstable
morphology
Neuroblast
products
Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus
Biochem/physiol Actions
Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.
Mouse C-1300 Neuroblastoma
Preparation Note
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
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