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Merck
CN

HF2x653

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

Human x Mouse Hybridoma hybrid

packaging

tube of 5 μg 90012609-DNA-5UG
pkg of vial of cells 90012609-1VL

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Fusion partner

Biochem/physiol Actions

Generated by electro-acoustic fusion of 2 lines. Parent: WI-L2-729-HF2. Murine myeloma: P3X63/Ag8.653. Non-immunoglobulin secreting, HAT-sensitive, resistant to ouabain at greater than 100uM, will fuse with normal human lymphocytes and lymphoblastoids.
Human (WI-L2-729-FF2) x mouse (P3X63/Ag8.653) heterohybridoma

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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