KC

Virus studies: rhabdovirus Drosophila X, picornavirus Drosophila C

Drosophila embryo

Resistant to osmolarity changes within 225-400 mosmols and to chloride levels 4-80mM; the usual Na/K ratio of 1.6 can be both increased and decreased considerably. Viruses present are non-pathogenic but can be induced when inoculated into a new host.

Maintain cultures between 3-9x100,000 cells/ml. Cells may adhere to the tissue culture flask, shake gently to dislodge; 25°C; No CO₂.

Schneider′s Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.