biological source
human skin
packaging
tube of 5 μg 90112604-DNA-5UG, pkg of vial of cells 90112604-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Genetic studies
Biochem/physiol Actions
Derived from a biopsy of an 11 year old Caucasian male with Cri du Chat syndrome. Cells have a limited lifespan of approximately 35 passages. Autoradiographic studies demonstrated that a distinction could be made between chromosome 4 and 5 in the Cri du chat syndrome by alternative DNA replication pattern and length of long and short arms.
Human Caucasian skin, Cri du Chat syndrome
Preparation Note
McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.