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About This Item
UNSPSC Code:
41106514
Biological source:
mouse lung
Growth mode:
Adherent
Karyotype:
Modal no. 64; hypodiploid
Morphology:
Fibroblast-like
biological source
mouse lung
packaging
tube of 5 μg 90112707-DNA-5UG, pkg of vial of cells 90112707-1VL
growth mode
Adherent
karyotype
Modal no. 64; hypodiploid
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies
Biochem/physiol Actions
Established from normal lung tissue of a new-born, ddY strain mouse. Both induced and spontaneous release of C-type particles have been reported.
Mouse ddY lung
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
动物来源培养基
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