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SW 1463

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, hypertriploid
Morphology:
Epithelial
Manufacturer/tradename:
ECACC
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biological source

human colon

manufacturer/tradename

ECACC

packaging

tube of 5 μg 90112713-DNA-5UG, pkg of vial of cells 90112713-1VL

growth mode

Adherent

karyotype

2n = 46, hypertriploid

morphology

Epithelial

products

Carcinoembryonic antigen (CEA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity studies

Biochem/physiol Actions

Human Caucasian rectum adenocarcinoma
Established from a grade II mucous producing and grade III solid rectal adenocarcinoma from a 66 year old Caucasian female. The cells form brush borders and produce CEA.
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16

Preparation Note

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split subconfluent cultures 1:2 using 0.25% trypsin or trypsin/EDTA; Medium change after 4-5 days. 37°C. No CO2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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