biological source
mouse blood
packaging
tube of 5 μg 91031103-DNA-5UG, pkg of vial of cells 91031103-1VL
growth mode
Suspension
karyotype
Modal no. 67-70
morphology
Lymphoblast
products
Immunoglobulin G (IgG2b), half molecules, light chain dimers, free light chains
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from the Merwin plasma cell tumour-II carried in BALB/c mice. Resembles the parent tumor in that they both produce fully assembled gamma globulin molecules (IgG2b) as well as half molecules, light chain dimers and free light chains. The MPC-11 produces 5-6 picograms of IgG2b per cell per minute.
Mouse BALB/C Myeloma
Preparation Note
DMEM + 2mM Glutamine + 20% Horse Serum (HS).
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.