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J774A.1 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91051511, mouse blood, Not specified

Synonym(s):

J774 A1 Cells, J774.A1 Cells, J774A1 Cells

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About This Item

UNSPSC Code:
41106514

Key Documents

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biological source

mouse blood

description

Mouse BALB/c monocyte macrophage

packaging

tube of 5 μg 91051511-DNA-5UG
pkg of vial of cells 91051511-1VL

growth mode

Semi-adherent

karyotype

Not specified

morphology

Not specified

products

Interleukin 1 (IL-1), lysozyme

receptors

Immunoglobulin, complement

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.
Metabolic studies

Biochem/physiol Actions

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.
Mouse BALB/c monocyte macrophage

Preparation Note

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm2 Incubate at 37°C; 5% CO2. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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