SK-OV-3 Cell Line human

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Human Caucasian ovary adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.