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About This Item
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Modal no. 47
Morphology:
Epithelial
biological source
human lung
packaging
tube of 5 μg 91091815-DNA-5UG, pkg of vial of cells 91091815-1VL
growth mode
Adherent
karyotype
Modal no. 47
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
NCI-H292 has been used to determine the ability of the cell line to support the replication of mumps virus.
Biochem/physiol Actions
Derived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. Cells retain mucoepidermoid characteristics in culture and support replication of Hepatitis B virus. Some of the cells contain numerous small mucin-containing granules and are L-DOPA decaroxylase negative. This cell line has been selected as a prototype for transfecting subgenome fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive and also stain positively for keratin and vimentin but negative for neurofilament triplet protein. Ethnicity: Black.
Human lung, mucoepidermoid carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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