biological source
human blood
packaging
tube of 5 μg 92111706-DNA-5UG, pkg of vial of cells 92111706-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Erythroid cell differentiation studies, induced globin synthesis
Biochem/physiol Actions
Derived from 30 year old male Caucasian. Expresses β-2 microglobulin and Ia antigen. Used in the study of erythroid cell differentiation and globin gene expression. Analogous to Friend cell leukaemia in mice. K-562 has similar characteristics.
Human Caucasian erythroleukaemia
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cell growth can be very slow on resuscitation. Stand the culture flask up overnight using 20% FBS.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.