COLO 677
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
human blood
packaging
tube of 5 μg 93072109-DNA-5UG
pkg of vial of cells 93072109-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Round & Refractile
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
COLO 677 was originally described as being derived from a tumour in the left axillary lymph node of a 39-year-old male with small lung cell carcinoma in 1989. However, DNA fingerprinting suggests cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old male with multiple myeloma in 1966 (see DSMZ website)
Human multiple myeloma
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Will form clusters in suspension.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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