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About This Item
UNSPSC Code:
41106514
Biological source:
human skin
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
biological source
human skin
packaging
tube of 5 μg 93082609-DNA-5UG, pkg of vial of cells 93082609-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Melanin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies
Biochem/physiol Actions
Granular fibroblasts. Derived from a human melanoma by Prof. C Grose (1978). The cells support growth of varicella-zoster virus (VZV) isolates at 36°C although viral growth is optimal at 32°C.
Human malignant melanoma
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA (0.5% trypsin in 0.53mM EDTA in modified Puck′s saline A); 5% CO2; 37°C.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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