biological source
rat pancreas
packaging
tube of 5 μg 93100618-DNA-5UG
pkg of vial of cells 93100618-1VL
growth mode
Semi-adherent aggregates
karyotype
Not specified
morphology
Pancreas cells
products
Amylase and exocrine enzymes
receptors
Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.
Biochem/physiol Actions
Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
Rat exocrine pancreatic tumour
Preparation Note
Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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