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216 BLCL human cell line

94031665, IHW Number: SLE

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biological source

human blood

ethnicity

Caucasian

gender

female

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

Not specified

Serological Profile

HLA A: 2 28
HLA C: 4 7
HLA B: 44 35
HLA DR: 2 11
HLA DQ: 1 3

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Regulatory Information

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