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About This Item
UNSPSC Code:
41106514
Biological source:
human blood
Technique(s):
cell culture | mammalian: suitable
Ethnicity:
French Canadian
Gender:
female
Growth mode:
Suspension
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 94050311-DNA-5UG, pkg of vial of cells 94050311-1VL
ethnicity
French Canadian
gender
female
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
A: 03 25
B: 47 37
HLA DRB1: 0101 1417
HLA DRB3: 0101
HLA DQA1: 0101 0103
HLA DQB1: 0501 0603
HLA DPA1: 1
HLA DPB1: 401
B: 47 37
HLA DRB1: 0101 1417
HLA DRB3: 0101
HLA DQA1: 0101 0103
HLA DQB1: 0501 0603
HLA DPA1: 1
HLA DPB1: 401
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
HLA A: 25 3
HLA C: 6
HLA B: 37 47
HLA DR: 1 6
HLA DR52: 52
HLA DQ: 1
HLA C: 6
HLA B: 37 47
HLA DR: 1 6
HLA DR52: 52
HLA DQ: 1
Regulatory Information
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