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About This Item
UNSPSC Code:
41106514
Biological source:
rat liver
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Hyperdiploid, 52 Chromosomes
Morphology:
Epithelial
packaging
pkg of vial of cells 94101906-1VL, tube of 5 μg 94101906-DNA-5UG
biological source
rat liver
growth mode
Adherent
karyotype
Hyperdiploid, 52 Chromosomes
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene expression analysis.
Biochem/physiol Actions
Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.
Rat hepatoma [HGPRT-]
Preparation Note
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm2 dish.
Other Notes
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Regulatory Information
低风险生物材料
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