HA cl. 55

Measles virus (strain Hallé)

Diagnosis, virus neutralisation.

Mouse BALB/c spleen x Mouse Sp2/0 Ag-14

The hybridoma cell line HA cl. 55 was produced by fusing myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. Immunofluorescence studies showed intracellular and membrane fluorescence with a diffuse pattern. The monoclonal antibody precipitates haemagglutinin polypeptide and neutralises measles virus infectivity. Biological assays showed haemagglutinin inhibition activity.

Maintain cultures between 1-5x100,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.