NP cl.25
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 95051114-DNA-5UG
pkg of vial of cells 95051114-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
Sp2/0 Ag-14
Reactivity
Anti-Measles Virus Nucleoprotein
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
Measles virus (strain Hallè)
Application
Diagnosis, study of measles virus infection
Biochem/physiol Actions
Mouse Sp2/0 x Mouse BALB/c spleen
NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.
Preparation Note
Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
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