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About This Item
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal no. 60
Morphology:
Epithelial
biological source
human lung
packaging
tube of 5 μg 95062823-DNA-5UG, pkg of vial of cells 95062823-1VL
growth mode
Adherent
karyotype
Modal no. 60
morphology
Epithelial
products
Variety of hormones, high level of calcitonin
receptors
Bombesin (BN), Epidermal growth factor (EGF), Transforming growth factor (TGF-beta), acetylcholine receptor (AChR)
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Human lung tumour model
Biochem/physiol Actions
DMS 53 is a small cell carcinoma of the lung (SCLC) isolated in 1974 from a mediastinal biopsy at time of diagnosis from a 54 year-old male patient prior to treatment. DMS 53 secrete a large variety of hormones including adrenocorticotropin (ACTH), bombesin (BN), calcitonin (CT), chorionic gonadotrophin (hCG), glucagon, growth hormone (GH), oxytocin-neurophysin (OT-NP), parathyroid hormone (PTH), somatostatin-like immunoreactivity (SRIF), thyroid releasing hormone (TRH) and 17β-estradiol. Expression of cell surface antigens Leu-7, Class I HLA, CLASS II HLA and My23 (defined by AML-2-23 monoclonal antibody) has been shown. DMS 53 cells produce receptors for BN, epidermal growth factor (EGF), transforming growth factor-β (TGF β) and acetylcholine (AChR). This cell line was shown to be tumourigenic in nude, athymic mice.
Human Caucasian lung small cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17
Preparation Note
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Other Notes
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Regulatory Information
常规特殊物品
低风险生物材料
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