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About This Item
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Polygonal
biological source
human lung
packaging
tube of 5 μg 95062832-DNA-5UG, pkg of vial of cells 95062832-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Calcitonin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Human lung tumour model, hormone secretion
Biochem/physiol Actions
Human Caucasian lung, small cell carcinoma
The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Regulatory Information
常规特殊物品
低风险生物材料
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