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About This Item
UNSPSC Code:
41106514
Biological source:
human lung
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Polygonal
biological source
human lung
packaging
tube of 5 μg 95062832-DNA-5UG, pkg of vial of cells 95062832-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Calcitonin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Human lung tumour model, hormone secretion
Biochem/physiol Actions
Human Caucasian lung, small cell carcinoma
The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Regulatory Information
常规特殊物品
低风险生物材料
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