PG Fish Ovary, ECACC 95070629

Fish Virology

Fish Northern Pike ovaries

The fish cell line PG has been initiated 1976 from ovarian tissue of 5 juvenile pike (Esox lucius). Cells grow at 4°C to 25°C with an optimum temperature of 20°C. PG cells are susceptible to various fish viruses including infectious pancreatic necosis viruses and pike fry rhabdovirus. The cell line has been mycoplasma eradicated at ECACC.

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week wit

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.