M-1
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95092201, mouse kidney, Epithelial-like
biological source
mouse kidney
packaging
tube of 5 μg 95092201-DNA-5UG
pkg of vial of cells 95092201-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Kidney cell studies, differentiation studies.
Biochem/physiol Actions
Derived from the cortical collecting duct (CCD) of a mouse, transgenic for the early region of SV40 (Tg(SV40E)Bri/7). They express many characteristics of the CCD like epithelial morphology and CCD-specific antigens. M-1 cells grown on permeable supports exhibited a high transepithelial resistance concomitant with the development of a lumen-negative transepithelial potential difference. The M-1 cell line and sub-clones exhibit principal cell (PC) functions and ß-intercalated cell (ß-ICC) functions, both typical for the heterogeneous epithelium found in the renal collecting duct. M-1 cells and sub-clones show a heterogeneous expression of PC and ß-ICC antigens with 5-10% exhibiting a dual PC/ß-ICC phenotype.
Mouse kidney cortical collecting duct, SV40 transformed
Preparation Note
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μm Dexamethasone (DXMT) + 5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Prolonged incubation with trypsin may be necessary for subculture.
Other Notes
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