P-tyr-1 Mouse x Mouse Hybridoma, ECACC 95092903

Aminobenzyl phosphonate

Detection of phosphotyrosine-containing proteins

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

The hybridoma cell line P-tyr-1 was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a female BALB/c mouse immunised with aminobenzyl phosphonate. Aminobenzyl phosphonate is a structural analogue of the side-chain in phosphotyrosine, and was coupled to hemocyanin (antibody P-HC) for immunisation. The secreted IgG1 antibodies react with phosphotyrosine and will detect phosphotyrosine-containing proteins by immunoprecipitation and Western blot analysis. The antibody does not react with phosphoserine or phosphothreonine. P-tyr-1 was tested for Bovine Viral Diarrhoea Virus (BVDV) at ECACC and found to be negative.

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.